Ankom TDF Dietary Uživatelský manuál Strana 1

Rev E 12/2/14 Operator’s Manual 2052 O’Neil Rd, Macedon NY 14502 Telephone: (315) 986-8090 Fax: (315) 986-8091 www.ankom.com

Operator’s Manual pg. 10 Rev E 12/2/14 Service Access Position If your instrument must be located in such a way as to restrict access to th

Operator’s Manual Rev E 12/2/14 pg. 11 Instrument Installation Site Requirements To install and operate the ANKOMTDF Dietary Fiber A

Operator’s Manual pg. 12 Rev E 12/2/14 1 – Pinch Valve Tubing Set (TDF71) 1 – Pump Tube Set (TDF68) 1 - Line Flush Tubing assy (TDF70) 1

Operator’s Manual Rev E 12/2/14 pg. 13 1 – Amylase/AMG Container assy (TDF57) (ONLY with AOAC 2009.01/2011.25 methods) 1 – Trizma Co

Operator’s Manual pg. 14 Rev E 12/2/14 7. Connect the Control Panel to the instrument. Remove the nuts from the right side of the instru

Operator’s Manual Rev E 12/2/14 pg. 15 10. Connect the Enzyme Containers to the corresponding ports. The Enzyme Containers conn

Operator’s Manual pg. 16 Rev E 12/2/14 Dietary Fiber Analysis Support Items The following support items needed to perform the fiber analysi

Operator’s Manual Rev E 12/2/14 pg. 17 IDF/SDF Analysis An IDF/SDF analysis measures the amount of IDF and SDF within a given sample

Operator’s Manual pg. 18 Rev E 12/2/14 6. Weigh Diatomaceous Earth (DE). DE is used during fiber analysis to enhance the flocculation and

Operator’s Manual Rev E 12/2/14 pg. 19 9.1.3 Press the ALL button to charge all of the lines. Each button will change color

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Operator’s Manual pg. 20 Rev E 12/2/14 10. Install SDF filter bags on the ANKOMTDF Dietary Fiber Analyzer. 10.1 Remove Clamp Bars A, B, C

Operator’s Manual Rev E 12/2/14 pg. 21 10.4 With the bag being held by the Delivery Nozzle, center it horizontally betwe

Operator’s Manual pg. 22 Rev E 12/2/14 11.4 Press the check mark () button on the Touch Screen Display. The following screen will be disp

Operator’s Manual Rev E 12/2/14 pg. 23 12.4 Place at least 20 mm (0.75 inches) of the filter section of the IDF bag inside the top

Operator’s Manual pg. 24 Rev E 12/2/14 13.2 Rinse the tin with no more than 3 ml of Distilled Water to ensure complete transfer. 13.3 Rep

Operator’s Manual Rev E 12/2/14 pg. 25 15. Verify that the fluid containers have the correct fluids for this procedure. Verify tha

Operator’s Manual pg. 26 Rev E 12/2/14 17. Connect the Nitrogen Supply to the instrument and turn on. Make sure the Nitrogen supply in you

Operator’s Manual Rev E 12/2/14 pg. 27 20. Start the instrument. The instrument is now compl

Operator’s Manual pg. 28 Rev E 12/2/14 21. Manually measure pH. One of the questions you are asked before starting the automated procedure

Operator’s Manual Rev E 12/2/14 pg. 29 22.6 With your Heat Sealer set between 3 and 4 (settings may vary depending on the heat seal

Table of Contents Introduction ...

Operator’s Manual pg. 30 Rev E 12/2/14 27. Determine the Protein content within the IDF and SDF residue. See the “Protein Determination” s

Operator’s Manual Rev E 12/2/14 pg. 31 TDF Analysis (AOAC 991.43) A TDF analysis directly measures the amount of TDF within a given

Operator’s Manual pg. 32 Rev E 12/2/14 6. Weigh Diatomaceous Earth (DE). DE is used during fiber analysis to enhance the flocculation and

Operator’s Manual Rev E 12/2/14 pg. 33 9.1.3 Press the ALL button to charge all of the lines. Each button will change co

Operator’s Manual pg. 34 Rev E 12/2/14 10. Install SDF filter bags on the ANKOMTDF Dietary Fiber Analyzer. 10.1 Remove Clamp Bars A, B, C

Operator’s Manual Rev E 12/2/14 pg. 35 10.4 With the bag being held by the Delivery Nozzle, center it horizontally betwe

Operator’s Manual pg. 36 Rev E 12/2/14 11.4 Press the check mark () button on the Touch Screen Display. The following screen will be disp

Operator’s Manual Rev E 12/2/14 pg. 37 12.4 Place at least 20 mm (0.75 inches) of the bottom of the IDF Flow-Thru bag inside the to

Operator’s Manual pg. 38 Rev E 12/2/14 13.3 Repeat steps 13.1 – 13.2 for all IDF stations. 13.4 Re-install Clamp Bar A by setting it on t

Operator’s Manual Rev E 12/2/14 pg. 39 15. Verify that the fluid containers have the correct fluids for this procedure. Verify tha

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Operator’s Manual pg. 40 Rev E 12/2/14 17. Connect the Nitrogen Supply to the instrument and turn on. Make sure the Nitrogen supply in you

Operator’s Manual Rev E 12/2/14 pg. 41 20. Start the instrument. The instrument is now compl

Operator’s Manual pg. 42 Rev E 12/2/14 21. Manually measure pH. One of the questions you are asked before starting the automated procedure

Operator’s Manual Rev E 12/2/14 pg. 43 23.6 With your Heat Sealer set between 3 and 4 (settings may vary depending on the heat seal

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Operator’s Manual Rev E 12/2/14 pg. 45 TDF Analysis (AOAC 985.29) A TDF analysis directly measures the amount of TDF within a given

Operator’s Manual pg. 46 Rev E 12/2/14 6. Weigh Diatomaceous Earth (DE). DE is used during fiber analysis to enhance the flocculation and

Operator’s Manual Rev E 12/2/14 pg. 47 9.1.3 Press the ALL button to charge all of the lines. Each button will change co

Operator’s Manual pg. 48 Rev E 12/2/14 10. Install SDF filter bags on the ANKOMTDF Dietary Fiber Analyzer. 10.1 Remove Clamp Bars A, B, C

Operator’s Manual Rev E 12/2/14 pg. 49 10.4 With the bag being held by the Delivery Nozzle, center it horizontally betwe

Operator’s Manual Rev E 12/2/14 pg. 5 Introduction ANKOM Technology designs, manufactures, and markets instruments and suppor

Operator’s Manual pg. 50 Rev E 12/2/14 11.4 Press the check mark () button on the Touch Screen Display. The following screen will be disp

Operator’s Manual Rev E 12/2/14 pg. 51 12.4 Place at least 20 mm (0.75 inches) of the bottom of the IDF Flow-Thru bag inside the to

Operator’s Manual pg. 52 Rev E 12/2/14 13.3 Repeat steps 13.1 – 13.2 for all IDF stations. 13.4 Re-install Clamp Bar A by setting it on t

Operator’s Manual Rev E 12/2/14 pg. 53 15. Verify that the fluid containers have the correct fluids for this procedure. Verify tha

Operator’s Manual pg. 54 Rev E 12/2/14 17. Connect the Nitrogen Supply to the instrument and turn on. Make sure the Nitrogen supply in you

Operator’s Manual Rev E 12/2/14 pg. 55 20. Start the instrument. The instrument is now compl

Operator’s Manual pg. 56 Rev E 12/2/14 21. Adjust pH before the Protease digestion. Just before the Protease digestion (after the bags hav

Operator’s Manual Rev E 12/2/14 pg. 57 24. Rinse the SDF filter bags with acetone. After the instrument has completed a TDF

Operator’s Manual pg. 58 Rev E 12/2/14 28. Determine the Ash content within the TDF residue. See the “Ash Determination” section of this m

Operator’s Manual Rev E 12/2/14 pg. 59 Protein Determination AOAC method 991.43 suggests that the Kjeldahl method be used for dete

Operator’s Manual pg. 6 Rev E 12/2/14 Method Comparison (Manual vs. Automated) AOAC 991.43 Method – IDF/SDF assay without automation Below

Operator’s Manual pg. 60 Rev E 12/2/14 SDF / TDF – Kjeldahl Method To determine the protein content within the SDF or TDF residue, follow t

Operator’s Manual Rev E 12/2/14 pg. 61 Ash Determination ANKOM Technology recommends that you use ceramic crucibles with

Operator’s Manual pg. 62 Rev E 12/2/14 SDF / TDF During a TDF procedure there is no residue captured in the IDF Flow-Thru bag. Therefore

Operator’s Manual Rev E 12/2/14 pg. 63 Productivity Enhancement Start a new procedure while a previous one is in progress To enhance

Operator’s Manual pg. 64 Rev E 12/2/14 To start a new assay immediately after an IDF process completes, follow the steps below. 1. Prepare

Operator’s Manual Rev E 12/2/14 pg. 65 3. Install new SDF bags on the ANKOMTDF Dietary Fiber Analyzer and continue the run. When an

Operator’s Manual pg. 66 Rev E 12/2/14 Expert Mode – save some set-up time At the beginning of each new run, the instrument gives the

Operator’s Manual Rev E 12/2/14 pg. 67 Status Screen The screen below shows the primary sections of the instrument Status screen

Operator’s Manual pg. 68 Rev E 12/2/14 7. Bottom process – Name Box #7 always displays “SDF” to represent an SDF process within an ID

Operator’s Manual Rev E 12/2/14 pg. 69 The values shown on this screen will dynamically change as the temperatures change. When the

Operator’s Manual Rev E 12/2/14 pg. 7 Instrument Description The ANKOMTDF Dietary Fiber Analyzer is designed to efficiently, accurat

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Operator’s Manual Rev E 12/2/14 pg. 71 Fault Handling When the instrument detects a problem, it generates a fault. Some faults requi

Operator’s Manual pg. 72 Rev E 12/2/14 # Name Description Result Correction E4 In-line Heater Over Temp The device that heats the fluid bef

Operator’s Manual Rev E 12/2/14 pg. 73 Diagnostics Mode To help solve problems that may occur while running the ANKOMTDF D

Operator’s Manual pg. 74 Rev E 12/2/14 The value on the Mixer Duty Cycle screen is only used during Diagnostics and in an AOAC 2009.01/20

Operator’s Manual Rev E 12/2/14 pg. 75 Motor Test When you press the Motor Test button on the Diagnostics screen, the following scre

Operator’s Manual pg. 76 Rev E 12/2/14 Digestion Times When you press the Digestion Times button the following screen will be displayed. T

Operator’s Manual Rev E 12/2/14 pg. 77 When you press one of the Continue buttons, the Pump Tube Test screen is once again displayed

Operator’s Manual pg. 78 Rev E 12/2/14 Clear IDF/SDF Lines When you press the Clear IDF/SDF Lines button on the Diagnostics screen, the IDF

Operator’s Manual Rev E 12/2/14 pg. 79 Service Mode – IDF Count When you press the IDF Count button on the Service Mode screen, the

Operator’s Manual pg. 8 Rev E 12/2/14 External Components – Left Side View External Components – Right Side View Control Panel (TDF17)

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Operator’s Manual Rev E 12/2/14 pg. 81 Periodic Maintenance Inspect and clean the delivery nozzles at each IDF and SDF station On a

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Operator’s Manual Rev E 12/2/14 pg. 83 QC / Calibration Checks The ANKOMTDF Dietary Fiber Analyzer is an automated and highly integr

Operator’s Manual pg. 84 Rev E 12/2/14 3. Press the Volume Calibration button. The following screen will be displayed. 4. Label and wei

Operator’s Manual Rev E 12/2/14 pg. 85 6. Install extra plastic containers under all six IDF Delivery Nozzles to catch any fluid cu

Operator’s Manual pg. 86 Rev E 12/2/14 When the fluid delivery is complete, the following screen will displayed. 9. Remove the beakers w

Operator’s Manual Rev E 12/2/14 pg. 87 5. Press the Main Menu button. The following screen will be displayed.

Operator’s Manual pg. 88 Rev E 12/2/14 8. Press the Temperature Sensor Corrections button. The following screen will be displayed. 9. P

Operator’s Manual Rev E 12/2/14 pg. 89 Appendix A – Reagents (AOAC 991.43, AOAC 985.29) Use Distilled Water throughout. Solutions co

Operator’s Manual Rev E 12/2/14 pg. 9 Internal Components – Rear View N2 Check Valve (1 of 12) Main Electrical Cabinet ID

Operator’s Manual pg. 90 Rev E 12/2/14 Appendix B – Instrument Out-of-use Procedure The fluid lines can become obstructed when the

Operator’s Manual Rev E 12/2/14 pg. 91 2. Open the pump doors. 2.1 Unscrew the six acorn nuts on the back of the instrument

Operator’s Manual pg. 92 Rev E 12/2/14 Appendix C – De-fatting Procedure According to the AOAC 985.29E method (and referred to in the AOAC

Operator’s Manual Rev E 12/2/14 pg. 93 5. Soak the bags in Petroleum Ether. 5.1 Place all XT4 Filter Bags into a beaker or other

Operator’s Manual pg. 94 Rev E 12/2/14 Appendix D – Accessories (sold separately) Contact ANKOM Technology or an authorized dealer to order

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Automation saves time and money! ANKOM Technology is an international company with products that include… A2000 Fiber Analyzer  Crude Fiber